Identification of Cross-linked Peptides Using Isotopomeric Cross-linkers
نویسندگان
چکیده
منابع مشابه
Quantitative cross-linking/mass spectrometry using isotope-labelled cross-linkers☆
UNLABELLED Dynamic proteins and multi-protein complexes govern most biological processes. Cross-linking/mass spectrometry (CLMS) is increasingly successful in providing residue-resolution data on static proteinaceous structures. Here we investigate the technical feasibility of recording dynamic processes using isotope-labelling for quantitation. We cross-linked human serum albumin (HSA) with th...
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Schafmeister, Po, and Verdine (another study) introduced a method using a hydrocarbon linker (staple) to stabilize a peptide in a helical configuration. One intended goal of this scheme is to facilitate the delivery of peptide drugs into target cells. Here, we investigate whether stapled peptides are intrinsically membrane permeable, by performing a case study on a stapled 12-mer peptide named ...
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Abstract This study focuses on realizations and discourse functions of the organizational linkers in the writing of research article abstracts from four disciplines. To this end, 120 research article abstracts from four disciplines namely, Applied Linguistics, Economics, Agriculture, and Applied Physics (30 from each discipline) were selected. All research article abstracts were extracted from ...
متن کاملCross-disciplinary use of Organizational Linkers in Research Article Abstracts
Abstract This study focuses on realizations and discourse functions of the organizational linkers in the writing of research article abstracts from four disciplines. To this end, 120 research article abstracts from four disciplines namely, Applied Linguistics, Economics, Agriculture, and Applied Physics (30 from each discipline) were selected. All research article abstracts were extracted from ...
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Mass spectrometric identification of cross-linked peptides can provide valuable information about the structure of protein complexes. We describe a straightforward database search scheme that identifies and assigns statistical confidence estimates to spectra from cross-linked peptides. The method is well suited to targeted analysis of a single protein complex, without requiring an isotope label...
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ژورنال
عنوان ژورنال: Journal of The American Society for Mass Spectrometry
سال: 2019
ISSN: 1044-0305,1879-1123
DOI: 10.1007/s13361-019-02253-z